Cutting the DNA
Über den Vortrag
Der Vortrag „Cutting the DNA “ von Georgina Cornwall, PhD ist Bestandteil des Kurses „Biotechnology“.
Quiz zum Vortrag
Which of the following is TRUE regarding the recombination process? Select all that apply.
- Sticky ends are the regions of recombinant DNA that are spliced together.
- Palindromic sequences in DNA are recognized by restriction endonucleases to create sticky ends.
- Type III restriction endonuclease is the most precise.
- Restriction enzymes are most commonly used to insert bacterial DNA into human DNA.
- Sticky ends have to be methylated to attach to each other.
Which of the following statements is INCORRECT regarding the EcoRI enzyme?
- The EcoRI enzyme helps eukaryotic cells to become immunized against viral attacks.
- EcoRI is a useful restriction endonuclease enzyme for gene-cloning procedures.
- The action of the EcoRI enzyme results in dsDNA fragments having sticky ends with 5′ end overhangs of AATT.
- EcoRI enzyme cuts the G/AATTC recognition sequence on the dsDNA that has a palindromic complementary sequence CTTAA/G.
- EcoRI was the first restriction endonuclease enzyme isolated from E. coli.
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Dozent des Vortrages Cutting the DNA
Georgina Cornwall, PhD
Dr. Georgina Cornwall is a Biology Instructor and currently works as an Instructional Designer in the private sector, developing interactive online training materials.
She obtained her PhD in Environmental, Population, and Organismic Biology at the University of Colorado, USA, in 2000, and has since taught a broad array of biology courses at various US universities.
She is an active promoter of hybrid/online teaching formats and interactive courses, and has received several awards for her engaging and innovative course design; e.g., she was named Adjunct Faculty of the Year at Colorado Mountain College in Aspen twice.
Within Lecturio, Dr. Cornwall teaches courses on Biology & Genetics.
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in depth knowledge on DNA splicing, ecor 1 and so the base pairs want to stick together and then ligase comes along to stick together the backbones, the phosphate-sugar backbones. Here you can see how the two pieces come together and now we have a recombinant DNA molecule. Again, we are generally looking at a circular chromosome and a foreign piece of DNA being inserted into that circular chromosome and so we'll see two ends of sticky ends and insertion of that sequence.
